Optimization of some parametric values of MTT for the determination of human melanoma (SK-Mel-30) cell viability

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazoliumbromide (MTT) assay is a widely used assessment method for the determination of anticancerogenic effects active compounds including plant secondary metabolites. Recently, some important ingredients have been investigated properties on melanoma cancer lines which are most lethal type skin cancer. Although methods DNA assay, 3H-thymidine incorporation and flow cytometry recommended counting cells in culture, MTT one frequent therefore, needs to be optimized cell lines. In this study, analytical procedure viability human line (SK-Mel-30) was divided into nine steps various parameters each step (reagent amount, incubation time, centrifugation, solvent type, waiting time before spectrophotometric analysis parameters) were optimized. Optimum amount reagent after addition determined as 10 µL 4 h 96 well plate, respectively. Various solvents evaluated solubility effectiveness formed crystals DMSO found best dissolve crystals. Waiting reading Uv-vis spectrums also evaluated. At end study flowchart, presented conditions, constructed. Obtained findings can ingredients.